ABSTRACT
Objective: To explore the expression of Dickkopf-3(DKK-3) mRNA in oral squamous cell carcinoma(OSCC) and the relationship between the promoter methylation status and the carcinogenesis of OSCC. Methods: The expression of DKK-3 gene in 51 cases of OSCC and corresponding normal mucosa tissue was detected by PT-PCR and methylation-specific PCR, respectively. The relationship between DKK-3 and the clinical pathological features of the patients was analyzed with SPSS 13. 0. Results: The expression level of DKK-3 in OSCC group was lower than that in the control(t =-12. 580, P＜ 0. 05). The methylation rate of DKK-3 gene promoter region in OSCC group was significantly higher than that in the control(χ2 = 19. 273, P＜ 0. 05). The mRNA expression level of DKK-3 gene in OSCC with methylation group was lower than that in the control(t =-2. 817, P＜ 0. 05). Conclusion: Down-regulation of DKK-3 gene expression and hypermethylation of promoter region are important mechanisms in the pathogenesis of OSCC. The hypermethylation of DKK-3 promoter may be the main cause of transcriptional silencing.
ABSTRACT
A case of eosinophilic lymphoid granuloma(ELG)was reported and the related literatures were reviewed.ELG is rare in clinic. The etiology and pathogenesis of ELG was unclear.The clinical feature includes enlarged lymph nodes which were always predilected for the head and neck regions,eosinophilic granulocytes and serum IgE rising.Lymphoid tissue hyperplasis formation of lymphoid follicles with active germinal centres are common in pathological examination.There is diffuse infiltration of eosinophils in interfollicular and perivascular zones. Surgery,drug therapy and radiotherapy are all effective for the treatment,but recurrence is often.
ABSTRACT
Objective:To observe the morphologic changes of cancer tissues and cells and clinical effect of microwave coagulation therapy (MCT) on oral squamous cell carcinoma.(OSCC), and then to evaluate the possibility of MCT in OSCC treatment.Methods:Forty OSCC patients were involved. MCT was adopted to the treatment of tumor primary focus, and associated with chemotherapy and lymphadenectomy of suprahyoid region or therapeutic neck dissection. After MCT the tissues were extracted for pathological examination immediately and morphologically observated by transmission electron microscope(TEM). All patients were received follow-up investigation for 3 years. The therapeutic effects of the two groups were observed and compared.Results:After MCT, the tissues of primary focus showed degenerative and necrotic tissue, inflammatory granulation, exudative necrosis, hyperplasia or atypical hyperplasia under light microscope. Apoptosis and mastocytes were observed besides the primary focus through TEM. Additionally MCT could protect oral faction, facies and improve survival quality and control local recurrence, which was more advanced than other old method (P